Development of a method for molecular subtyping <i>Bacillus anthracis</i> using HRM PCR

Introduction. Bacillus anthracis is the causative agent of anthrax, a pathogen characterized by high genetic monomorphism that complicates differentiation strains. Thus, molecular methods for typing require improvement.
 The aim study. To select marker SNPs new groups B. and to develop method their laboratory identification using HRM PCR.
 Materials methods. core genome 222 strains from GenBank database 66 collection pathogenic microorganisms Stavropol Anti-Plague Institute was aligned parsnp software. A dendrogram based on 7242 built in MEGA X validation included representatives various groups. PCR reaction performed "Type-it Kit" "KAPA FAST qPCR Rotor Gene DNA thermocycler with function. Data analysis visualization were custom scripts Python R development environments.
 Results discussion. Marker 6 have been identified, which make it possible determine whether belong one 7 subclusters. Pairs primers selected loci containing them, parameters optimized discrimination different alleles SNP loci, an scheme developed.
 Conclusion. subclusters A.Br.CEA, A.Br.STI, A.Br.Tsiankovskii, B.Br.Europe, B.Br.Siberia, B.Br.Asia, B.Br.018, developed subtyping PCR.